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Bioinformatic and Phylogenetic Investigation of WRKY Genes Involved in Drought Stress in Camelina sativa Plant | ||
| Agrotechniques in Industrial Crops | ||
| دوره 4، شماره 2، شهریور 2024، صفحه 65-79 اصل مقاله (1.4 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22126/atic.2023.8830.1084 | ||
| نویسندگان | ||
| Seyede Maryam Seyed Hassan Pour1؛ Leila NejadSadeghi* 1؛ Danial Kahrizi2؛ Zahra Sadat Shobbar3 | ||
| 1Department of Genetic and Plant Breeding, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Camelina (Camelina Sativa) is a hexaploid dicotyledonous plant from the Brassicaceae family, which is very similar to the Arabidopsis. The number of camelina chromosomes is 40=2n. WRKY transcription factors are one of the most important gene families in plants that play an important role in regulating growth and development and response to various stresses. In this research, 224 WRKY genes were identified in the camelina plant by searching the database, and the chromosomal position, gene length, and conserved motifs were identified in the camelina plant based on the Arabidopsis. Also, in this study, in order to validate the research, 2 genes WRKY8 and WRKY57 under drought stress were investigated by the qPCR method. The results indicated that both the above-mentioned genes were strongly expressed under drought stress conditions in tolerant varieties compared to normal conditions, but the trend was opposite in sensitive varieties. This study has provided acceptable and valuable information for studying the evolution and function of the WRKY gene family in camelina. | ||
تازه های تحقیق | ||
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| کلیدواژه‌ها | ||
| Abiotic stress؛ qRT-PCR؛ Brassicaceae؛ Transcriptional factors؛ WRKY | ||
| مراجع | ||
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