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Sorbitol MacConkey agar and wastewaters` coliphages | ||
Journal of Applied Research in Water and Wastewater | ||
دوره 10، شماره 2 - شماره پیاپی 20، اسفند 2023، صفحه 112-118 اصل مقاله (686.63 K) | ||
نوع مقاله: Research Paper | ||
شناسه دیجیتال (DOI): 10.22126/arww.2024.9527.1305 | ||
نویسندگان | ||
Tesfaye Legesse Bedada* 1؛ Teshome Belachew Eshete2؛ Adey Feleke Desta3؛ Fitsum Tigu Yifat3؛ Dagim Jirata Birri3؛ Kassu Desta Tullu4؛ Samson Girma Gebre1؛ Firehiwot Abera Dera1؛ Kaleab Sebsibe Awoke1؛ Tatek Kasim Feto1؛ Waktole Gobena Sima1؛ Elias Seyoum Deribe5؛ Dejenie Teklu Teklu5؛ Getinet Fikresilasie Irena1؛ Geremew Tassew Weledesemayat1؛ Tigist Yohannis Negassi1؛ Asnake Desalegn Garedew Garedew3 | ||
1Public Health Microbiology Research Team, Ethiopian Public Health Institute, Addis Ababa, Ethiopia. | ||
2Gondar University, Gondar, School of Biomedical and Laboratory Sciences, Ethiopia. | ||
3Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia. | ||
4Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa. University, Ethiopia. | ||
5Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian Public Health Institute, Ethiopia. | ||
چکیده | ||
A total of 114 samples collected from hospital wastewaters and rivers in Addis Ababa, Ethiopia were tested for non-sorbitol fermenting bacteria and coliphages. Sorbitol MacConkey agar is mainly used in the detection of E. coli O157:H7. However, other emerging diarrhoeagenic enteropathogens such as Plesiomonas shigelloides, Edwardsiella tarda, Providencia alcalifaciens, Escherichia albertii, Escherichia vulneris and Escherichia fergusonii were detected in the samples using this medium. Information for most of the emerging enteropathogens is scarce in most countries including Ethiopia. A total of 20 different genera, 38 species of non-sorbitol fermenting bacteria were isolated. Escherichia coli O157 could not be detected from any of the samples. All these backgrounds may mask the detection of Escherichia coli O157. Even if sorbitol MacConkey agar has several background limitations, different emerging diarrhoeagenic non-sorbitol fermenting bacteria were detected in the majority of the rivers and hospitals` wastewaters samples. The correlation between coliphages and non-sorbitol fermenting bacteria were not significant. As several bacteria have been isolated on sorbitol MacConkey agar medium, it is essential that the most selective laboratory techniques will be desired for outbreak investigation of E. coli O157, but other non-sorbitol fermenting enteropathogens should also be detected using sorbitol MacConkey agar in low resources countries. | ||
کلیدواژهها | ||
Coliphage؛ Emerging diarrhoeagenic bacteria؛ Hospitals wastewater؛ Sorbitol MacConkey agar؛ Non-sorbitol fermenting bacteria؛ Rivers | ||
مراجع | ||
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