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Proteomic Profiling and Antioxidant Enzyme Activity Response to Drought Stress in Bread Wheat (Triticum aestivum) Rijaw Cultivar | ||
| بیوتکنولوژی و بیوشیمی غلات | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 21 مهر 1404 | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22126/cbb.2025.11971.1105 | ||
| نویسندگان | ||
| مهدی کاکائی* 1؛ علی مصطفایی2؛ محسن سعیدی3 | ||
| 1گروه علوم کشاورزی دانشگاه پیام نور، تهران-ایران. | ||
| 2Professor, Medical Biology Research Center, University of Medical Sciences, Kermanshah-Iran. | ||
| 3گروه مهندسی تولید و ژنتیک گیاهی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه ، ایران. | ||
| چکیده | ||
| Introduction: Drought is one of the significant factors limiting wheat yield (Triticum aestivum L.). Understanding the morphological and physiological aspects of drought resistance is crucial in mitigating the problems caused by drought conditions. Drought limits photosynthesis and ultimately causes plant death; therefore, research on drought is beneficial. Drought stress plays a crucial role in physiological processes, metabolism, and the expression of several genes related to plant adaptation to drought stress. Materials and Methods: To evaluate the effect of drought on protein profile, bread wheat (Rijaw cv) seedlings were grown in pots in a greenhouse. Drought stress was applied after the tillering stage and before the beginning of the stem stage, and its amount was considered to be 30% of Field Capacity. Leaf tissue samples were harvested 12 days after stress and immediately after being separated from the plant. Then, they were placed in aluminum foil and stored in liquid nitrogen until extraction. To compare the changes in leaf protein profiles between drought-treated and control plants, a proteomics method was employed. Leaf proteins were separated using the TCA-Acetone method and two-dimensional gel electrophoresis in the pH range of 4-10. Results: Out of more than 400 protein spots reproducibly detected and analyzed, 29 spots showed significant changes under drought. Some proteins showed genotype-specific patterns of up- or down-regulation in response to drought. According to the results of variance analysis, chlorophyll a, chlorophyll b, and total chlorophyll were significantly different (P < 0.01) between the control sample and the other stress conditions, indicating a difference between the plants in the experimental treatments. Additionally, in terms of soluble protein traits, a significant difference exists between the treatments (P < 0.01), and this difference is evident in the expression of proteins across all treatments. Regarding the studied enzymes, peroxidase, superoxide dismutase, and carotenoids, a significant difference was observed between all the applied treatments (P < 0.01) and for the catalase enzyme (P < 0.05). Conclusion: The results of mean comparison of all studied traits confirmed the significant difference; the data observed in this research showed that changes in antioxidant enzymes could provide a model to show the drought tolerance of wheat cultivars. Moreover, this research was able to identify the proteins involved in the tolerance of Rijaw cultivar wheat seedlings to drought stress, underscoring the importance of understanding the role of antioxidant enzymes in drought tolerance. Keywords: Drought, 2D Electrophoresis, Peroxidase, Superoxide dismutase, Catalase, Carotenoids. | ||
| کلیدواژه‌ها | ||
| Drought؛ 2D Electrophoresis؛ Peroxidase؛ Superoxide dismutase؛ Catalase؛ Carotenoids | ||
| مراجع | ||
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